Determination of chlorogenic acid and ferulic acid in shenghuagao electuary by HPLC

Objective A method was established for determination of the content of chlorogenic acid and ferullic acid in Shenghuagao electuavy by HPLC.Methods The separation was performed on a Kromasil C18 column with the mobile phase consisting of acetonitrile-1% glacialacetic acid water for gradient elution.Chlorogenic acid and ferulic acid were determined by dual wavelength of 323 nm(λ1)and 327 nm(λ2).Results The linearities were in the range of 0.058～0.812 mg/mL for chlorogenic acid,and 0.48～6.72 μg/mL for ferulic acid;the average recovery was 100.4% and 98.99%,RSD was 0.9%.Conclusion The method is simple and rapid.It can be used for quality control in production of Shenghuagao electuavy.