Determination of Three Constituents in Ginsenoside-Sodium Chloride Injection By HPLC

Aim To establish a method to control the quality of Gisenoside-Sodium chloride injection.Methods The three constituents of ginsenoside Rb 1,ginsenoside Rg 1,notoginsenoside R 1 were determined by HPLC method.Chromatographic conditions included Shim-pack CLC-ODS colun,mobile phase using of Acetonitrile and 0.05% Phosphoric acid (35∶65) for determining ginsenoside Rb 1,Acetonitrile and 0.05% Phosphoric acid (25∶75) for determining ginsenoside Rg 1 and notoginsenoside R 1.The flow rate was 1.5ml/min.Detection wavelength was 203nm.Results A good linearity was obtained respectively in the range of 0.79～3.94μg for ginsenoside Rb 1,0.62～3.21μg for ginsenoside Rg 1 and 0.65～3.57μg for notoginsenoside R 1.The average recovery rates of ginsenoside Rb 1,ginsenoside Rg 1 and notoginsenoside R 1 were 100.14% (RSD=0.73%),99.66% (RSD=0.72%),100.99%(RSD=0.59%),respectively.Conclusion The method appeared to be simple,quick,sensitive,accurate,reproducible and could be used for the quality control of Gisenoside-Sodium chlonide injection.