ScholarLens Back to search
2007•Zhongguo yaofangRequires access

Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Sanqishangyao Tablets by HPLC

Wenzhong Zhang, Fu Jia Yu

Open publisher page 0 citations

Abstract

OBJECTIVE:To set up a HPLC method for the determination of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao tablets.METHODS:It was performed on VP-ODS (150mm×4.6mm,5μm),and the mobile phase was acetonitrile-water with gradient elution system(0～20min(20∶80),20～26min(40∶60),26～35min(20∶80)).The flow rate was 1.0mL·min-1 and the column temperature was room temperature.The detection wavelength was 203nm.RESULTS:The liner range of notoginsenoside R1 was 0.261～7.83μg(r=0.999 9)and the average recovery was (101.12±2.03)%(RSD=2.1%).The liner range of ginsenoside Rg1 was 0.602 5～18.075μg(r=0.999 7)and the average recovery rate was (97.22±3.33)%(RSD=1.9%).The liner range of ginsenoside Rb1 was 0.537 5～16.125μg(r=0.999 6)and the average recovery rate was(100.71±2.4)%(RSD=1.6%).CONCLUSION:The method is suitable for the content determination of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao Tablets.

About this research paper

What this paper is about

OBJECTIVE:To set up a HPLC method for the determination of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao tablets.METHODS:It was performed on VP-ODS (150mm×4.6mm,5μm),and the mobile phase was acetonitrile-water with gradient elution system(0～20min(20∶80),20～26min(40∶60),26～35min(20∶80)).The flow rate was 1.0mL·min-1 and the column temperature was room temperature.The detection wavelength was 203nm.RESULTS:The liner range of notoginsenoside R1 was 0.261～7.83μg(r=0.999 9)and the average recovery was (101.12±2.03)%(RSD=2.1%).The liner range of ginsenoside Rg1 was 0.602 5～18.075μg(r=0.999 7)and the average recovery rate was (97.22±3.33)%(RSD=1.9%).The liner range of ginsenoside Rb1 was 0.537 5～16.125μg(r=0.999 6)and the average recovery rate was(100.71±2.4)%(RSD=1.6%).CONCLUSION:The method is suitable for the content determination of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao Tablets.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE:To set up a HPLC method for the determination of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao tablets.METHODS:It was performed on VP-ODS (150mm×4.6mm,5μm),and the mobile phase was acetonitrile-water with gradient elution system(0～20min(20∶80),20～26min(40∶60),26～35min(20∶80)).The flow rate was 1.0mL·min-1 and the column temperature was room temperature.The detection wavelength was 203nm.RESULTS:The liner range of notoginsenoside R1 was 0.261～7.83μg(r=0.999 9)and the average recovery was (101.12±2.03)%(RSD=2.1%).The liner range of ginsenoside Rg1 was 0.602 5～18.075μg(r=0.999 7)and the average recovery rate was (97.22±3.33)%(RSD=1.9%).The liner range of ginsenoside Rb1 was 0.537 5～16.125μg(r=0.999 6)and the average recovery rate was(100.71±2.4)%(RSD=1.6%).CONCLUSION:The method is suitable for the content determination of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao Tablets.

Key concepts: Ginsenoside Rg1, Chromatography, Ginsenoside, Chemistry, Gradient elution, High-performance liquid chromatography, Recovery rate, Content determination

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Sanqishangyao Tablets by HPLC — Research Paper | ScholarLens