Determination of notoginsenoside R_1 and ginsenoside Rg_1,Rb_1 in rat plasma by HPLC

OBJECTIVE To establish an assay method for the determination of notoginsenoside R1 and ginsenoside Rg1、Rb1 in rat plasma.METHODS Icariin was used as the internal standard.C18 column was used.The mobile phase was acetonitrile-water gradient elution,flow rate was 1.0 mL·min-1,the column temperature was 30 ℃ and the detected wavelength was at 203 nm.RESULTS Three saponins were separated well.A good linear relationship was obtained with the range of 2-200 μg·mL-1 for notoginsenoside R1,5.5-550 μg·mL-1 for ginsenoside Rg1 and 10.5-1050 μg·mL-1 for ginsenoside Rb1.The limit detection was 0.6,0.55,0.79 μg·mL-1,respectively.CONCLUSION The method is simple and accurate for analysis of notoginsenoside R1 and ginsenoside Rg1、Rb1 in rat plasma.