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Determination of the Content of Adenosine in Xuezhitong Capsule by HPLC

SU Yan-wen

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Abstract

Objective To establish a determination method of the content of adenosine in Xuezhitong Capsule by HPLC.MethodsAgilent C18 column(4.6 mm×250 mm,5μm PN 990967-902,SNUSNH008613,LNBO5096,made in USA) was used.For the determination of the content of adenosine,the mobile phase was pH 6.5 liquid of both 0.02% acetic acid glacial with triethylamine and methanol(92∶8),and the detection wavelength was 260nm,column temperature was 28℃.ResultsThe calibration curve of adenosine was linear over the range of was 0.0105~0.3144μg.The correlation coefficient was 0.999 99(n=7).The average recovery of adenosine was 100.1%,RSD=1.81%(n=6).ConclusionThe method is accurate,simple and highly repeatable.

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What this paper is about

Objective To establish a determination method of the content of adenosine in Xuezhitong Capsule by HPLC.MethodsAgilent C18 column(4.6 mm×250 mm,5μm PN 990967-902,SNUSNH008613,LNBO5096,made in USA) was used.For the determination of the content of adenosine,the mobile phase was pH 6.5 liquid of both 0.02% acetic acid glacial with triethylamine and methanol(92∶8),and the detection wavelength was 260nm,column temperature was 28℃.ResultsThe calibration curve of adenosine was linear over the range of was 0.0105~0.3144μg.The correlation coefficient was 0.999 99(n=7).The average recovery of adenosine was 100.1%,RSD=1.81%(n=6).ConclusionThe method is accurate,simple and highly repeatable.

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Available abstract

Objective To establish a determination method of the content of adenosine in Xuezhitong Capsule by HPLC.MethodsAgilent C18 column(4.6 mm×250 mm,5μm PN 990967-902,SNUSNH008613,LNBO5096,made in USA) was used.For the determination of the content of adenosine,the mobile phase was pH 6.5 liquid of both 0.02% acetic acid glacial with triethylamine and methanol(92∶8),and the detection wavelength was 260nm,column temperature was 28℃.ResultsThe calibration curve of adenosine was linear over the range of was 0.0105~0.3144μg.The correlation coefficient was 0.999 99(n=7).The average recovery of adenosine was 100.1%,RSD=1.81%(n=6).ConclusionThe method is accurate,simple and highly repeatable.

Key concepts: Adenosine, Calibration curve, Chromatography, High-performance liquid chromatography, Triethylamine, Capsule, Acetic acid, Content determination

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