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Determination of Adenosine in Ganoderma Capsule by RP-HPLC

LI Kai-zhi

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Abstract

OBJECTIVE:To establish a RP-HPLC method for the determination of Adenosine in Ganoderma capsule.METHODS:HPLC method was adopted with Diamonsil C18(150mm× 4.6mm,5μ m)at a temperature of 30℃.The mobile phase consisted of methanol-0.01mol· L-1 KDP solution(10∶ 90)at a flow rate of 0.8mL· min-1.A UV-detector at 260nm was used.RESULTS:The linear range of Adenosine was 0.081 4～ 2.036 0μ g(r=0.999 4).The average recovery was 99.37%(RSD=0.23%,n=6).CONCLUSION:The method is accurate,simple,rapid,and reliable,and suitable for the quality control of Ganoderma capsule.

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OBJECTIVE:To establish a RP-HPLC method for the determination of Adenosine in Ganoderma capsule.METHODS:HPLC method was adopted with Diamonsil C18(150mm× 4.6mm,5μ m)at a temperature of 30℃.The mobile phase consisted of methanol-0.01mol· L-1 KDP solution(10∶ 90)at a flow rate of 0.8mL· min-1.A UV-detector at 260nm was used.RESULTS:The linear range of Adenosine was 0.081 4～ 2.036 0μ g(r=0.999 4).The average recovery was 99.37%(RSD=0.23%,n=6).CONCLUSION:The method is accurate,simple,rapid,and reliable,and suitable for the quality control of Ganoderma capsule.

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Available abstract

OBJECTIVE:To establish a RP-HPLC method for the determination of Adenosine in Ganoderma capsule.METHODS:HPLC method was adopted with Diamonsil C18(150mm× 4.6mm,5μ m)at a temperature of 30℃.The mobile phase consisted of methanol-0.01mol· L-1 KDP solution(10∶ 90)at a flow rate of 0.8mL· min-1.A UV-detector at 260nm was used.RESULTS:The linear range of Adenosine was 0.081 4～ 2.036 0μ g(r=0.999 4).The average recovery was 99.37%(RSD=0.23%,n=6).CONCLUSION:The method is accurate,simple,rapid,and reliable,and suitable for the quality control of Ganoderma capsule.

Key concepts: Ganoderma, Capsule, Chromatography, High-performance liquid chromatography, Chemistry, Adenosine, Linear range, Ganoderma lucidum

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