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2010•Journal of Traditional Chinese Veterinary MedicineRequires access

Determination of adenosine contents in Banqing granule by HPLC

Fan Chuan-yuan

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Abstract

Objective:To establish a method for the determination of adenosine contents in Banqing granules.Methods:HPLC was used by chromatographic column of ODS (4.6 mm×250 mm,15 cm),detective wavelength at 260 nm,the mobile phase of methanol-water (10:90),column worked at room temperature and the flow rate at 1.0 mL/min.Results:It present a good linear relationship when the sample size of adenosine was within the range of 0.162-1.619 μg,with relationship coefficient of 0.999 5,and the average recovery was 99.02%,RSD was 0.82%(n=5).Conclusion:The method is convenient,rapid and accurate.

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What this paper is about

Objective:To establish a method for the determination of adenosine contents in Banqing granules.Methods:HPLC was used by chromatographic column of ODS (4.6 mm×250 mm,15 cm),detective wavelength at 260 nm,the mobile phase of methanol-water (10:90),column worked at room temperature and the flow rate at 1.0 mL/min.Results:It present a good linear relationship when the sample size of adenosine was within the range of 0.162-1.619 μg,with relationship coefficient of 0.999 5,and the average recovery was 99.02%,RSD was 0.82%(n=5).Conclusion:The method is convenient,rapid and accurate.

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Available abstract

Objective:To establish a method for the determination of adenosine contents in Banqing granules.Methods:HPLC was used by chromatographic column of ODS (4.6 mm×250 mm,15 cm),detective wavelength at 260 nm,the mobile phase of methanol-water (10:90),column worked at room temperature and the flow rate at 1.0 mL/min.Results:It present a good linear relationship when the sample size of adenosine was within the range of 0.162-1.619 μg,with relationship coefficient of 0.999 5,and the average recovery was 99.02%,RSD was 0.82%(n=5).Conclusion:The method is convenient,rapid and accurate.

Key concepts: Chromatography, Adenosine, Granule (geology), Linear relationship, High-performance liquid chromatography, Chemistry, Linear range, Methanol

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