Cloning and sequencing of Glycoprotein cDNA of Rabies virus Flury strain

According to GenBank of the rabies virus glycoprotein gene sequence,two pairs of specific primers were designed using Oliga software.Amplified the full-length cDNA fragment of GP gene of Flury strain by reverse transcription-polymerase chain reaction(RT-PCR)and cloned into pMD-18T vector for sequencing.Sequence and homology analysis showed that the cDNA of RGP encoding 524 amino acids was consisted of 1 574 nucleic acids.The homologies of nucleic acid and amino acid sequences to those reported were 82.3%~96.3% and 88.4%~94.3%,respectively.