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Cloning and sequence analysis of glycoprotein gene of rabies virus.

Gao Ming-hua, Zhiyan Zhang, Fan QingDe, Yang XiaoGang, Xianzhu Xia

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Abstract

According to nucleoprotein gene sequence of Rabies virus(RV) accessed by GenBank,a pair of specific primers was designed to amplify G gene of RV strain ERA by RT-PCR.The PCR product was purified and cloned into pMD18-T.The positive recombinant pMD-G was used for sequencing.The results of sequencing showed that the complete length of G gene was 1 575 bp,which encoded 524 amino acids.The homologies of nucleic acid and deduced amino acid sequence of glycoprotein compared with the standard strains(CVS,PV,SRV9,SAD-B19)were 89.1%～99.1% and 89.7%～99.3%,respectively.There are three potential N-glycosylation sites in deduced G protein.The analysis of hydrophobicity,Jameson-Wolf antigenic epitopes and surface probability plot of G proteins of ERA showed that it was as well as with CVS,PV and other strains.

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What this paper is about

According to nucleoprotein gene sequence of Rabies virus(RV) accessed by GenBank,a pair of specific primers was designed to amplify G gene of RV strain ERA by RT-PCR.The PCR product was purified and cloned into pMD18-T.The positive recombinant pMD-G was used for sequencing.The results of sequencing showed that the complete length of G gene was 1 575 bp,which encoded 524 amino acids.The homologies of nucleic acid and deduced amino acid sequence of glycoprotein compared with the standard strains(CVS,PV,SRV9,SAD-B19)were 89.1%～99.1% and 89.7%～99.3%,respectively.There are three potential N-glycosylation sites in deduced G protein.The analysis of hydrophobicity,Jameson-Wolf antigenic epitopes and surface probability plot of G proteins of ERA showed that it was as well as with CVS,PV and other strains.

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Available abstract

According to nucleoprotein gene sequence of Rabies virus(RV) accessed by GenBank,a pair of specific primers was designed to amplify G gene of RV strain ERA by RT-PCR.The PCR product was purified and cloned into pMD18-T.The positive recombinant pMD-G was used for sequencing.The results of sequencing showed that the complete length of G gene was 1 575 bp,which encoded 524 amino acids.The homologies of nucleic acid and deduced amino acid sequence of glycoprotein compared with the standard strains(CVS,PV,SRV9,SAD-B19)were 89.1%～99.1% and 89.7%～99.3%,respectively.There are three potential N-glycosylation sites in deduced G protein.The analysis of hydrophobicity,Jameson-Wolf antigenic epitopes and surface probability plot of G proteins of ERA showed that it was as well as with CVS,PV and other strains.

Key concepts: Rabies virus, GenBank, Biology, Gene, Recombinant DNA, Nucleoprotein, Cloning (programming), Epitope

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