Cloning and sequencing of N gene of strain M of rabies virus isolated from mouse

The nucleoprotein structural gene of rabies virus M strain was amplified by RT-PCR from genomic RNA and cloned into pGEM-T plasmid vector and sequenced.Comparison and analysis of the nucleotide sequence and amino acid sequence with those of other 9 strains previously published were performed by computer with DNAStar software.The results showed that the nucleotide homology of them ranged from 71.0 % to 99.8 % and deduced amino acid sequence homology from 77.6 % to 99.6 %.Both cDNA sequence and the deduced amino acid sequence homology of strain M with CVS are the highest and with Mokola are the lowest.The nucleotide sequence homology of strain M with CTN is lower.The results play an important role for the investigation of molecular epidemiology and the study on looking for suitable genetic engineering vaccine against rabies virus.