Cloning and Sequencing of Glycoprotein Gene of Chinese Rabies Vaccine Strain CTN-1-V

Objective To study the structure of glycoprotein (GP) gene of Chinese rabies vaccine strain CTN-1-V. Methods Amplify the full-length cDNA fragment of GP from the Vero cells infected with CTN- 1-V strain by RT-PCR and clone into PCR2.1 vector for sequencing. Results The cDNA of GP of CTN-1-V strain consisted of 1 575 nucleic acids encoding 524 amino acids. The homologies of nucleic acid and ami-no acid sequences to those reported abroad were 80.8%-92.4% and 82.9%-93.3% respectively. Conclusion The study provided a theoretical basis for selecting a candidate vaccine strain.