Effect of bFGF on Autogenous Nerve Transplantation

Objective To expole the effect of bFGF on autogenous nerve transplantation.Methods A segment about 10 mm of the right sciatic never was severed in each of the 10 adult rats which were divided into 2 groups equally.The experimental group(AF) and control group(A).The epineurium of the severed nerve segments were sutured in original situs.bFGF of 100u was injected in group AF while only normal saline was injected in group A.The rats survived for twelve weeks after autografting.Results After treated and calculated with biosterology,it was found that the regenerating nerve fibers grew through the proximal suture into the autograft,then into the distal segment of the sciatic under light-microscope,while the absolute value,revival rate and stereological parameters such as facial number density(NA)、facial facial density(AA) of the regenerating nerve were significantly higer in group AF than those in group A.The absolute value,revival rate,volume density and number density of the neurons in the anterior horn of L4-6 spinal segments and ganglia were significantly higher in group AF than those in group A.Conclusion The bFGF not only protects the cell body of neuron,but also promotes the regeneration of peripheral nerve.