Effects of bFGF complex degradation membrane on allograft of nerves

Objective To investigate the effects of bFGF complex degradation membrane on the regeneration of nerve. Methods Sixty rats were randomly divided into 3 groups. Two-centimeter sciatic nerve of the left lower limb was cut off in all rats. The nerve segments were then stored in -80 ℃ for 10 d for allograft transplantation. In group A, the nerve segments were treated with bFGF solution and coated with bFGF membrane before allograft transplantion. In group B, the nerve segments were without bFGF treatment and only coated with membrane not containing bFGF. In group C, the nerve segments were without any treatment. A series of examinations were conducted at 90 d and 180 d after transplantation. Results In group A, the amount and diameter of sheathed nerve fiber was larger than that in group B and C (P0.05). The connective tissue was less in group A. The electrophysiological parameters and the restoration of blood-nerve barrier in group A were better than those in group B and C. Conclusion bFGF might promote the regeneration of nerve allograft by interfering connective tissue proliferation.