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Determination of Encapsulation Efficiency and Release Rate of Baicalin Liposome by UV Spectrophotometry

WU Wen-ying

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Abstract

Objective To determine the encapsulation efficiency and the release rate of baicalin liposome. Methods The baicalin was separated with Sephadex G-50, and determined by ultraviolet spectrophotometry. Results Baicalin liposome had a good linearity within the range of 4-20 μg·mL -1 ( r =0.999 4, n =6), the average recovery was 98.83%, RSD =2.26% . Conclusion This method is simple, accurate which can be used for the determination of encapsulation efficiency and release rate of baicalin liposome.

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Objective To determine the encapsulation efficiency and the release rate of baicalin liposome. Methods The baicalin was separated with Sephadex G-50, and determined by ultraviolet spectrophotometry. Results Baicalin liposome had a good linearity within the range of 4-20 μg·mL -1 ( r =0.999 4, n =6), the average recovery was 98.83%, RSD =2.26% . Conclusion This method is simple, accurate which can be used for the determination of encapsulation efficiency and release rate of baicalin liposome.

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Available abstract

Objective To determine the encapsulation efficiency and the release rate of baicalin liposome. Methods The baicalin was separated with Sephadex G-50, and determined by ultraviolet spectrophotometry. Results Baicalin liposome had a good linearity within the range of 4-20 μg·mL -1 ( r =0.999 4, n =6), the average recovery was 98.83%, RSD =2.26% . Conclusion This method is simple, accurate which can be used for the determination of encapsulation efficiency and release rate of baicalin liposome.

Key concepts: Baicalin, Liposome, Chromatography, Chemistry, Spectrophotometry, Recovery rate, Sephadex, High-performance liquid chromatography

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