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2013•Journal of Practical Traditional Chinese Internal MedicineRequires access

Determinations of the Contents of Puerarin in Guanmaikang Capsule by HPLC

MA Yueha

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Abstract

[Objective]To develop a HPLC method for the determination of puerarin in Guanmaikang capsule.[Methods]The analysis was carried out on Eclipse×DB-C18. The mobile phase was consisted of MeOH-H20(25 :75);The flow rate was 1.0 mL/min;the detective wave was 250 nm and the column temperature was set at 25℃.[Results]The calibration curves of puerarin was in good linearity over the range of 40.04ng ~ 240.24ng,The linear equation was y=2.649+4.325×105x,r=0.9991;The average recovery was96.56%,RSD was 1.1 %. The contents of puerarin in 5 batches samples were determined,and the puerarin content in Guanmaikang capsule should not be less than 8.0 mg/ capsules.[Conclution]The method is simple,rapid,reliable and can be used for the Guanmaikang capsule quality contro1.

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What this paper is about

[Objective]To develop a HPLC method for the determination of puerarin in Guanmaikang capsule.[Methods]The analysis was carried out on Eclipse×DB-C18. The mobile phase was consisted of MeOH-H20(25 :75);The flow rate was 1.0 mL/min;the detective wave was 250 nm and the column temperature was set at 25℃.[Results]The calibration curves of puerarin was in good linearity over the range of 40.04ng ~ 240.24ng,The linear equation was y=2.649+4.325×105x,r=0.9991;The average recovery was96.56%,RSD was 1.1 %. The contents of puerarin in 5 batches samples were determined,and the puerarin content in Guanmaikang capsule should not be less than 8.0 mg/ capsules.[Conclution]The method is simple,rapid,reliable and can be used for the Guanmaikang capsule quality contro1.

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Available abstract

[Objective]To develop a HPLC method for the determination of puerarin in Guanmaikang capsule.[Methods]The analysis was carried out on Eclipse×DB-C18. The mobile phase was consisted of MeOH-H20(25 :75);The flow rate was 1.0 mL/min;the detective wave was 250 nm and the column temperature was set at 25℃.[Results]The calibration curves of puerarin was in good linearity over the range of 40.04ng ~ 240.24ng,The linear equation was y=2.649+4.325×105x,r=0.9991;The average recovery was96.56%,RSD was 1.1 %. The contents of puerarin in 5 batches samples were determined,and the puerarin content in Guanmaikang capsule should not be less than 8.0 mg/ capsules.[Conclution]The method is simple,rapid,reliable and can be used for the Guanmaikang capsule quality contro1.

Key concepts: Puerarin, Capsule, Chromatography, High-performance liquid chromatography, Calibration curve, Content determination, Medicine, Biomedical engineering

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