ScholarLens Back to search
2009•Strait Pharmaceutical JournalRequires access

Determination of puerarin in Xingkeshu capsule by HPLC

Baojun Li

Open publisher page 0 citations

Abstract

OBJECTIVE To determine the contents of the puerarin in Xingkeshu capsule by HPLC.METHODS RP-HPLC was used with a column of ODS and a mobile phase of methanol-water(25∶75),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 20μL.RESULTS The retention time of the puerarin was about 13.25min,the puerarin was within 0.2～3.2μg·mL-1.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 99.98%(n=5,RSD=1.13%).CONCLUSION This method was accurate,reliable and can be used to control puerarin in Xingkeshu capsule.

About this research paper

What this paper is about

OBJECTIVE To determine the contents of the puerarin in Xingkeshu capsule by HPLC.METHODS RP-HPLC was used with a column of ODS and a mobile phase of methanol-water(25∶75),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 20μL.RESULTS The retention time of the puerarin was about 13.25min,the puerarin was within 0.2～3.2μg·mL-1.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 99.98%(n=5,RSD=1.13%).CONCLUSION This method was accurate,reliable and can be used to control puerarin in Xingkeshu capsule.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To determine the contents of the puerarin in Xingkeshu capsule by HPLC.METHODS RP-HPLC was used with a column of ODS and a mobile phase of methanol-water(25∶75),the flow rate was 1mL·min-1,the detection wavelength was 250nm and the column temperature was 25℃,the injection volume was 20μL.RESULTS The retention time of the puerarin was about 13.25min,the puerarin was within 0.2～3.2μg·mL-1.The correlation coefficient was 0.9999.The average recoveries of paeoniflorin were 99.98%(n=5,RSD=1.13%).CONCLUSION This method was accurate,reliable and can be used to control puerarin in Xingkeshu capsule.

Key concepts: Puerarin, Paeoniflorin, Chromatography, High-performance liquid chromatography, Chemistry, Capsule, Correlation coefficient, Mathematics

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of puerarin in Xingkeshu capsule by HPLC — Research Paper | ScholarLens