Comparison of Four Methods for Genomic DNA Extraction from Aphis glycines

Using Aphis glycines as material,four genomic DNA extraction method including chloroform-isoamyl alcohol extraction,salting-out method,KAc and genomic DNA kit method were compared and analyzed.The purity of genomic DNA from chloroform-isoamyl alcohol extraction and salting-out method was better than the other two methods.And the total time of extraction in ascending order was genomic DNA kit method,salting-out method,the chloroform-isoamyl alcohol extraction and KAc.Salting-out method acted more quickly and effectively,the other three methods also had some advantages,therefore,the corresponding methods for genomic DNA extraction should be chosen according to the test conditions.