Comparing the Three Methods to Extract Human Genomic DNA from Whole Blood

Objective: To explore a rapid,economical method for extracting high quality genomic DNA expectably,the differences of improved phenol-chloroform method,salt-extraction method and kit method on extracting human genomic DNA from whole blood were investigated.Methods: Human genomic DNA was extracted by these three methods,respectively.The genomic DNA yield,purity and quality were measured by ultraviolet photometer,agarose gel electrophoresis,PCR amplification and digesting of restrictive endonuclease.Results: The genomic DNA yield of improved phenol-chloroform method,salt-extraction method and kit method were 125.36± 9.39 ng.μL-1,97.26±19.89 ng.μL-1,383.14±23.01 ng.μL-1,respectively.However,genomic DNA was not integrity with the kit method,and there was no significant difference in DNA purity between the improved phenol-chloroform method and the kit method.The salt-extraction method delivered significantly lower yields of DNA and DNA purity compared to other two methods.Moreover,PCR amplification was not stable with genomic DNA extracted by using the salt-extraction method.The genomic DNA extracted with these three methods were all identified by digesting of restrictive endonuclease effectively.Conclusion: Improved phenol-chloroform method is an economical,rapid,efficient and stable method for extracting genomic DNA from whole blood,and is applicable for large numbers of clinical samples.