Comparative Study of Two Cattle Genomic DNA Extraction Methods

The genomic DNA extraction method,cooking extraction method in water,was studied and the comparison with the traditional phenol and chloroform method was done.The cooking extraction method was the method from which genomic DNA was obtained when the cells were broken and proteins were denatured after the cells were cooked and cooled quickly.The feasibility and integrality of the extracted genomic DNA were detected through agrose gel electrophoresis and PCR amplification.During detection,the PCR amplification of the extracted genomic DNA was done with 3 pairs of primers and the genomic DNA was also amplified after preserved at-20 ℃.The analysis result indicated that cooking extraction method is a quick,simple,economic and efficient method for genomic DNA extraction.