2012•Zhongguo redai yixueRequires access

Comparison of three methods for human genomic DNA extraction for long-term stored whole blood

Cai Wang-wei

Abstract

Objective To choose a proper methods for genomic DNA extraction in order to build a genomic DNA library.Method The efficiency of three genomic DNA extraction methods including modified phenol-chloroform method,SDS method and Kit method were compared.Genomic DNA was extracted using each genomic DNA extraction method and detected by PCR.Result The extraction efficiency of three DNA extraction methods had significant statistical difference(P0.01).The extraction efficiency of modified phenol-chloroform method was the highest,however,SDS and Kit method extraction methods were low.Conclusion is Modified phenol-chloroform method was recommended for human genomic DNA extraction in order to build a genomic DNA library.

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Key concepts: genomic DNA, DNA, DNA extraction, Extraction (chemistry), Chromatography, Chloroform, Biology, Computational biology