Effects of Curcumin,12-Tetradecanoyl-Phorbol-13-Acetate on Cell Growth of Human Ovarian Cancer Cell Line SKOV3

Objective:To study the effects of Curcumin(CCM)、12-tetradecanoyl-phorbol-13-acetate(TPA) on the cell proliferation,morphology and cell cycle of human ovarian cell line SKOV3,to explore the inhibition mechanism of the human ovarian cancer cell line SKOV3.Methods:Growth inhibition rates of SKOV3 cells were measured by MTT method.Morphological changes of cells were observed under fluorescent invert microscope and electromicroscope.Cell cycle was checked by flow cytometery(FCM).Results:CCM can inhibit cell proliferation of SKOV3 in a dose and time dependent manner,growth were hindered at stage G1/S.Partial cells presented the characteristic morphological changes of apoptosis.TPA can inhibit cell proliferation of SKOV3 cell in a does and time dependent manner.Growth were arrested at stage G1→S.Conclusions:CCM and TPA can significantly inhibit the growth of SKOV3 in vitro by inhibiting the cell proliferation and arrest the cell cycle.