Apoptosis Induced by FTY720 in Human Ovary Carcinoma Cell Line SKOV3

Objective To investigate the growth-inhibitory and apoptosis-inducing effect of FTY720 on human ovary carcinoma cell line SKOV3 and its mechanism in vitro.Methods We collect SKOV3 cells at 24h and 48h respectively under different concentration of FTY720.The inhibition rates of cells were determined by methyl thiazolyl tetrazolium(MTT) assay to evaluate the effect of FTY720.The percentage of apoptosis and cell cycle progression were detected by flow cytometry(FCM).Caspase proteins were determined by western-blot.Results Growth inhibition and apoptosis induction were evoked by different concentrations of FTY720 in SKOV3 cells in time-and concentration-dependent manner.FTY720 greatly inhibited human ovary cancer cell SKOV3 proliferation.This effect was associated with G1 phase cell cycle arrest and apoptosis.Western-blot showed that caspase 3,caspase 9 and PARP were activated.Conclusion FTY720 can inhibit the proliferation and induce SKOV3 cells apoptosis.Apoptosis induced by FTY720 may be regulated through G1 phase cell cycle arrest and the activation of endogenous caspase pathway.