Effects of 12-tetradecanoyl-phorbol-13-acetate on the morphology and proliferation of human ovary cancer cell line SKOV3

[Objective] This study was designed to explore the effects of TPA on the proliferation,morphology,cell cycle distribution and ultrastructure of human ovary cell line SKOV3.[Methods] Grouth inhibition rates of SKOV3 cells were measured with MTT method.Cell cycle distribution and apoptosis was determined by Flow-cytometry.Morphological changes and ultramicrostructure of cells were observed with inverted phase contrast microscope and transmission electron microscope.[Results] TPA could inhibited cell proliferation of human ovary cancer SKOV3 cells in a does-and time-dependent manner.[Conclusions]TPA can inhibite cell proliferation of human ovary cancer skov3 cells and significantly inhibit the growth of ovary cancer cells.