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Determinationof cyclamate in the liquor with high-performance liquid chromatography (HPLC) method

Shen Guo-jun

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Abstract

The HPLC method was used to determine the cyclamate contents in the liquor. Chromatography column: ODS Hypersil (5μm, 125 mm×4 mm), mobile phase: CH3OH:H2O = 80:20, flow rate: 1.0 mL, detection wavelength: 314 nm. The results showed that linear range 4:μg/mL-100μg/mL, recycling rate: 80.6%-102.7%, RSD: 10.8%-2.5%, lower limit of quantitative detection: 1.2μg/mL, higher limit of quantitative detection: 4.0μg/mL. This method is convenient and reliable, and is suitable for the detection of cyclamate contents in the liquor.

About this research paper

What this paper is about

The HPLC method was used to determine the cyclamate contents in the liquor. Chromatography column: ODS Hypersil (5μm, 125 mm×4 mm), mobile phase: CH3OH:H2O = 80:20, flow rate: 1.0 mL, detection wavelength: 314 nm. The results showed that linear range 4:μg/mL-100μg/mL, recycling rate: 80.6%-102.7%, RSD: 10.8%-2.5%, lower limit of quantitative detection: 1.2μg/mL, higher limit of quantitative detection: 4.0μg/mL. This method is convenient and reliable, and is suitable for the detection of cyclamate contents in the liquor.

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Available abstract

The HPLC method was used to determine the cyclamate contents in the liquor. Chromatography column: ODS Hypersil (5μm, 125 mm×4 mm), mobile phase: CH3OH:H2O = 80:20, flow rate: 1.0 mL, detection wavelength: 314 nm. The results showed that linear range 4:μg/mL-100μg/mL, recycling rate: 80.6%-102.7%, RSD: 10.8%-2.5%, lower limit of quantitative detection: 1.2μg/mL, higher limit of quantitative detection: 4.0μg/mL. This method is convenient and reliable, and is suitable for the detection of cyclamate contents in the liquor.

Key concepts: Chromatography, Detection limit, High-performance liquid chromatography, Chemistry, Linear range, Volumetric flow rate, Quantum mechanics, Physics

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