HPLC Determination of the Content of Celifixne
Teng Wang
Abstract
Teng Wang
Abstract
Method:To determine the content of celifixine by HPLC, the HPLC system consisted C18 column (6.0×150mm), (340:160) mixture as mobile phase ,with detection at 254 nm.Result: The linear range was 0.144 mg/ml~0.280 mg/ml (r=0.9999),The mean recovery was 99.8%,RSD= 0.20%(n=3). The method is simple,sensitive and accurate.
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Method:To determine the content of celifixine by HPLC, the HPLC system consisted C18 column (6.0×150mm), (340:160) mixture as mobile phase ,with detection at 254 nm.Result: The linear range was 0.144 mg/ml~0.280 mg/ml (r=0.9999),The mean recovery was 99.8%,RSD= 0.20%(n=3). The method is simple,sensitive and accurate.
Key concepts: High-performance liquid chromatography, Chromatography, Linear range, Chemistry, Content determination, Detection limit