2007•Lishizhen Medicine and Materia Medica ResearchRequires access
Study on the Separation and Purification of Total Flavone from Herb Cirsii japonici by Macroporous Absorption Resin
Lai Xue-wen
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Abstract
Objective To select the suitable resin and establish the optimal conditions for separation and purification of total flavone from herb Cirsii japonici.Methods To determine the optimal one by inspection of four types of macroporous resin.The optimal process of purification was studied by single factor analysis.Results The static absorption capacity,the absorption ratio and the static elution ratio of AB-8 resin was higher than the others .The optimal technological conditions were found in AB-8 as follows:sample concentration was 5.2 mg/ml,flow rate was 0.5 ml/min,sample volume was 3.5BV,and 80% ethanol was used as eluting agent and its dosage was 10BV.Conclusion The type of AB-8 showed comprehensive property in the condition and the yield of the total flavones achieved nearly 90%.