Study on the Separation and Purification of Total Flavone from Semen Litchi by Macroporous Absorption Resin

Objective :To select the suitable resin and establish the optimal conditions for separation and purification of total flavone from Semen Litchi.Methods :To determine the optimalone by inspection of four types ofmacroporous resin.The optimal process of purification was studied by single factor analysis.Results : The static absorption capacity,the absorption ratio and the static elution ratio of D4006resin was higher than the others.The optimal technological conditions were found in D4006 as follows: sample concentration was 5.45 mg/mL,flow rate was 0.5 mL/min,sample volume was 4 BV,and 80% ethanol was used as eluting agent and its dosage was 14 BV.Conclusion :The type of D4006 showed comprehensive property in the condition and the yield of the total flavones achieved nearly 90%.