Purification of Total Flavones from Valeriana jatamansi by Macroporous Resins

Objective:To study the optimal technologies for the purification of total flavones from Valeriana jatamansi with macroporous resin.Method: Taking the static elution ratio and absorption ratio as indexes,the static absorption-desorption was used and compared in order to find the optimum macroporous resin.The technical process for purification of total flavones with the optimum macroporous resin was screened by yield of total flavones as index.Result: The HPD600 macroporous resin among five types of resins was found with the best separating efficiency,by which static absorption capacity and desorption ratio could reach 131.11 mg·g-1 and 91.69%,respectively.The best result of dynamic absorption was based on the followings: sample concentration 3.42 g·L-1,feed rate 2.4 BV·h-1,sample capacity 25.64 mg·g-1,elution flow rate 2.4 BV·h-1.After eluted with 3BV of distilled water and 4 BV of 80% ethanol,the yield of total flavones was 80.36% and the purity was 41.20%.Conclusion: With definite technical process,the HPD600 macroporous resin could be used to purify the total flavonoids of V.jatamansi.