Effects of Linggui Zhugan Decoction on NF-κB in Rats with Ventricular Remodeling after Acute Myocardial Infarction

Objective:To study the mechanism of Linggui Zhugan decoction(LGZGD)interference on ventricular remodeling(VR) in rats after acute myocardial infarction(AMI),through observing the expression of nuclear factor-κB(NF-κB)and NF-κB mRNA of myocardial tissue and the content of NF-κB in ventricular remodeling rats with AMI.Method: AMI model was produced by ligation of coronary artery.2 weeks after modeling,rats were randomly classified into model,captopril,and low(2.1 g · kg-1),middle(4.2 g · kg-1) and high(8.4 g · kg-1)dosage of LGZGD group.Control group and the other 5 groups were administered medications intragastrically for 4 consecutive weeks.The content of NF-κB in myocardial tissue and serum was detected by ELISA and the expression of NF-κB was assayed by RT-PCR and the expression of NF-κB mRNA by Western blot.Result: For sham-operation,model,low,middle and large dosage of LGZGD group and captopril group,the content of NF-κB of myocardial tissue was 0.190±0.011,0.772±0.026,0.366±0.059,0.295±0.033,0.235±0.013,0.341±0.023.The relative expression of NF-κB mRNA were 1.000,26.875,6.574,4.340,1.194,5.540.The content of serum NF-κB were(125.85±14.76),(196.98±17.79),(163.89±20.08),(131.73±10.47),(141.93±10.33),(133.93±9.27)ng·L-1.The content of NF-κB in serum and the expression of NF-κB,NF-κB mRNA of myocardial tissue of model group increased significantly compared with control group(P0.01).Low,middle and high dosage of LGZGD and captopril group could decrease the content of NF-κB in serum and inhibited the expression of NF-κB,NF-κB mRNA in myocardial tissue of model rats significantly compared with model group(P0.01 or P0.05).Conclusion: The mechanism of LGZGD interference VR of post-AMI seems to be related to the inhibition of NF-κB.