Effects of rosuvastatin on protein expression in myocardial tissue of rats with acute myocardial infarction

Objective To investigate rosuvastatin on protein expression in myocardial tissue of rats with acute myocardial infarction(AMI). Methods Female SD rats were selected and established the rat model of AMI by ligation of the left anterior descending coronary artery(LAD). Fifty-four qualified rats were randomly divided into rosuvastatin groups with dosages of 10 6 mg/kg and the model group. And another 18 rats with Sham operations were selected into the control group. Rats in rosuvastatin groups were ig administered with rosuvastatin(adding into distilled water) with dosages of 10 and 6 mg/kg according to clinical common dosages, but the other Sham and model groups were ig administered with 0.9% saline solution. The treatment were adopted once daily, and lasted for 4 weeks. Protein expression difference of Periostin, nuclear factor κB(NF-κB), and interleukin-6(IL-6) in myocardial tissue of rats were detected by immunohistochemistry method, expression of Periostin, NF-κB, IL-6, and TGF-β1 m RNA were detected by RT-PCR, and collagen volume fraction(CVF) of rats in each group were compared. Results Compared with model group, Periostin value and positive index(PI) of NF-κB, and IL-6 in rosuvastatin groups were obviously decreased(P 0.05), and expressions of Periostin, NF-κB, IL-6, and TGF-β1 m RNA were obviously decreased(P 0.05), and CVF value was also significantly reduced(P 0.05). Conclusion Rosuvastatin can regulate protein expressions or m RNA expressions of Periostin, NF-κB, IL-6, and TGF-β1 to improve cardiac remodeling of rats with AMI.