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2006•Chinese Journal of Hospital PharmacyRequires access

Determination of quercetin in compound flavone capsules by HPLC

Fan Lv-lin

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Abstract

OBJECTIVE To establish a method for determining the content of quercetin in compound flavone capsules.METHODS The suntek 18 column with methanol- 0.4% phosphorous acid (45∶55) as the mobile phase was used.The flow rate was 0.9 mL·min -1and the detective wavelength was 360 nm.RESULTS The linear range of calibration curve was 0.324 8- 3.248 0 μg,for the quercetin (r= 0.999 8).The average recovery was 99.13% and the RSD was 0.78% (n= 6).CONCLUSION The method is simple,and accurate with a good reproducibility.

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OBJECTIVE To establish a method for determining the content of quercetin in compound flavone capsules.METHODS The suntek 18 column with methanol- 0.4% phosphorous acid (45∶55) as the mobile phase was used.The flow rate was 0.9 mL·min -1and the detective wavelength was 360 nm.RESULTS The linear range of calibration curve was 0.324 8- 3.248 0 μg,for the quercetin (r= 0.999 8).The average recovery was 99.13% and the RSD was 0.78% (n= 6).CONCLUSION The method is simple,and accurate with a good reproducibility.

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Available abstract

OBJECTIVE To establish a method for determining the content of quercetin in compound flavone capsules.METHODS The suntek 18 column with methanol- 0.4% phosphorous acid (45∶55) as the mobile phase was used.The flow rate was 0.9 mL·min -1and the detective wavelength was 360 nm.RESULTS The linear range of calibration curve was 0.324 8- 3.248 0 μg,for the quercetin (r= 0.999 8).The average recovery was 99.13% and the RSD was 0.78% (n= 6).CONCLUSION The method is simple,and accurate with a good reproducibility.

Key concepts: Quercetin, Calibration curve, Reproducibility, Chromatography, Chemistry, High-performance liquid chromatography, Methanol, Content determination

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