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2001•Journal of Anhui Traditional Chinese Medical CollegeRequires access

CONTENT DETERMINATION OF QUERCETIN IN QUERCETIN CAPSULE BY HPLC METHOD

Zheng Zhi

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Abstract

Objective: To develop a HPLC method for the determination of quercetin in Quercetin capsule.Method: the method has been carried out by using the ultrasphere Kromasil column (4.6 mm×250 mm, 5μm) and methanol phosphate buffer solution(1 000 ml H 2O+4 ml H 3PO 4+2 ml TEA)(60∶40) as mobile phase.A flow rate of 1.0 ml/min and the detection wavelength of 373 nm were adopted. Result: A satisfactory separation among Quercetin and related impurities was obtained. The calibration curve was linear in the range of 7～70 μg/ml for Quercetin .The average recovery of Quercetin was 99.47%(RSD=0.97%). Conclusion: This method is simple, rapid,specific,and can be used for the preparation as a Quercetin quality control standard.

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What this paper is about

Objective: To develop a HPLC method for the determination of quercetin in Quercetin capsule.Method: the method has been carried out by using the ultrasphere Kromasil column (4.6 mm×250 mm, 5μm) and methanol phosphate buffer solution(1 000 ml H 2O+4 ml H 3PO 4+2 ml TEA)(60∶40) as mobile phase.A flow rate of 1.0 ml/min and the detection wavelength of 373 nm were adopted. Result: A satisfactory separation among Quercetin and related impurities was obtained. The calibration curve was linear in the range of 7～70 μg/ml for Quercetin .The average recovery of Quercetin was 99.47%(RSD=0.97%). Conclusion: This method is simple, rapid,specific,and can be used for the preparation as a Quercetin quality control standard.

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Available abstract

Objective: To develop a HPLC method for the determination of quercetin in Quercetin capsule.Method: the method has been carried out by using the ultrasphere Kromasil column (4.6 mm×250 mm, 5μm) and methanol phosphate buffer solution(1 000 ml H 2O+4 ml H 3PO 4+2 ml TEA)(60∶40) as mobile phase.A flow rate of 1.0 ml/min and the detection wavelength of 373 nm were adopted. Result: A satisfactory separation among Quercetin and related impurities was obtained. The calibration curve was linear in the range of 7～70 μg/ml for Quercetin .The average recovery of Quercetin was 99.47%(RSD=0.97%). Conclusion: This method is simple, rapid,specific,and can be used for the preparation as a Quercetin quality control standard.

Key concepts: Quercetin, Chromatography, Chemistry, Calibration curve, High-performance liquid chromatography, Capsule, Linear range, Detection limit

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