Influence factor on the rules of duplicate examination of Sysmex-1800i blood analyzer for platelet count

Objective:To discuss and establish proper duplicate examination rules of Sysmex-1800i blood analyzer for platelet count. Methods:We used Sysmex-1800i blood analyzer to detect 450 blood samples which were divided into three groups,group of red blood cell with different MCV,group of platelet with different internal factor and varied methods of taking blood group. Counted the platelet by manual under the microscope and reviewed by blood smear. Results:①MCV≥ 80 fl and 70 fl≤MCV80 fl,there was no difference between instrument and hand for platelet count (P0.05),but when 60 fl≤ MCV70 fl,the difference existed (P0.05). MCV60 fl,also,there was difference between the two methods (P0.05). ②Difference was observed between the methods of instrument and hand when big platelet or abnormal platelet histogram (P0.05). When platelet aggregation there was significantly different between platelet count measured by equipment and blood smear. ③If the results of blood analysis could be accepted under different methods of taking blood,it has advantages of stable results,plenty of samples and easy to review by using the EDTA-k2 vacuum blood collection tube. Venous blood,while giving priority to the clinical situation can select the sample type and blood containers. When thrombopenia was detected,the venous blood should be taken again and count the platelet by hands. Conclusion:MCV≤70 fl of the red blood cell,the samples was not qualified,the results and the clinical findings do not match,it is necessary to count the platelet by hands.