2012•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access
Determination of Paeonolum and Paeoniflorin in Jinsangqingyin Pills by HPLC
Hualong Chen
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Abstract
Objective To establish a method for determination of paeonolum and paeoniflorin in Jinsangqingyin pills by HPLC.Methods HPLC was applied on a Hypersil ODS2(5 μm,4.6 mm×200 mm) column.The mobile phase for paeonolum consisted of methanol and water(55∶ 45) at a detection wavelength of 274 nm.The flow rate was 1.0 ml · min-1.The mobile phase for paeoniflorin consisted of acetonitrile and 0.033 mol · L-1 potassium dihydrogen phosphate solution(15∶ 85) at a detection wavelength of 230 nm and a flow rate of 1 ml · min-1.Results The linear range of paeonolumand paeoniflorin was 0.0427-0.6835 μg(r=0.9997)and 0.2084-3.3344 μg(r=0.9998).The average recovery of paeonolumand paeoniflorin was 98.7% and 98.6%.RSD was 0.73% and 0.96%.Conclusion This method is simple,accurate,reproducible and suitable for quality control of this drug.