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2013•Acta Horticulturae SinicaRequires access

Cloning and Functional Analysis of OguCMS-related Gene BoMF1 Promoter in Brassica oleracea

Guo Ying-ying

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Abstract

The regulative sequence ( 521 bp )of OguCMS-related gene BoMF1 promoter from Brassica oleracea was cloned by genomic walking (TAIL-PCR). In silico analysis showed that this sequence contained several acting elements,including TATA-box and CAAT-box,MYB binding sites, phytohormone responsive elements and so on. In order to study the promoter function,a 521 bp promoter sequence of BoMF1 was inserted upstream of the GUS reporter gene replacing the CaMV35S promoter of pBI121. The plant expression vector pBI121-BoMF1P and pBI121 were transformed into Arabidopsis thaliana with the Agrobacterium tumefaciens strain LBA4404. It was suggested that pBI121-BoMF1P could drive the GUS gene exclusively express in anther and pollen of Arabidopsis thaliana.

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What this paper is about

The regulative sequence ( 521 bp )of OguCMS-related gene BoMF1 promoter from Brassica oleracea was cloned by genomic walking (TAIL-PCR). In silico analysis showed that this sequence contained several acting elements,including TATA-box and CAAT-box,MYB binding sites, phytohormone responsive elements and so on. In order to study the promoter function,a 521 bp promoter sequence of BoMF1 was inserted upstream of the GUS reporter gene replacing the CaMV35S promoter of pBI121. The plant expression vector pBI121-BoMF1P and pBI121 were transformed into Arabidopsis thaliana with the Agrobacterium tumefaciens strain LBA4404. It was suggested that pBI121-BoMF1P could drive the GUS gene exclusively express in anther and pollen of Arabidopsis thaliana.

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Available abstract

The regulative sequence ( 521 bp )of OguCMS-related gene BoMF1 promoter from Brassica oleracea was cloned by genomic walking (TAIL-PCR). In silico analysis showed that this sequence contained several acting elements,including TATA-box and CAAT-box,MYB binding sites, phytohormone responsive elements and so on. In order to study the promoter function,a 521 bp promoter sequence of BoMF1 was inserted upstream of the GUS reporter gene replacing the CaMV35S promoter of pBI121. The plant expression vector pBI121-BoMF1P and pBI121 were transformed into Arabidopsis thaliana with the Agrobacterium tumefaciens strain LBA4404. It was suggested that pBI121-BoMF1P could drive the GUS gene exclusively express in anther and pollen of Arabidopsis thaliana.

Key concepts: Biology, Gene, Agrobacterium tumefaciens, CAAT box, Arabidopsis thaliana, Genetics, Reporter gene, Promoter

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Cloning and Functional Analysis of OguCMS-related Gene BoMF1 Promoter in Brassica oleracea — Research Paper | ScholarLens