The expression and significance of the multidrug resistance-related genes MDR1、MRP、LRPmRNA and their proteins P-gp、MRP、LRP in human non-small cell lung cancer tissues

Objective:To investigate the expression and significance of the multidrug resistance-related genes multidrug resistance gene1(MDR1)、multidrug resistance-related protein(MRP)、lung resistance protein(LRP)mRNA and their proteins P-glycoprotein(P-gp)、MRP、LRP in human non small cell lung cancer(NSCLC)tissues and paratumor tissues.Methods:To examine the expression level of MDR1、MRP、LRP mRNA and their proteins P-gp、MRP 、 LRP in 43 samples of NSCLC and in 15 samples of paratumor tissues by immunohistochemistry(IHC)and reverse transcription polymerase chain reaction(RT-PCR).Results:The expression rates of MDR1、MRP and LRP mRNA were 79.06%(34/43)、65.17%(28/43)、86.05%(37/43)and 20.00%(3/15)、13.33%(2/15)、13.33%(2/15)in 43 tumor tissues and in 15 paratumor tissues,respectively.The expression rates of P-gp、MRP and LRP were 74.42%(32/43)、67.44%(29/43)、88.37%(38/43)and 13.33%(2/15)、20.00%(3/15)、6.67%(1/15)in 43 tumor tissues and in 15 paratumor tissues,respectively.There was statistic difference in the expression of MDR1、MRP、 LRPmRNA and their corresponding proteins(P-gp、MRP、LRP)between lung cancer tissues and paratumor tissues(P0.05).Coexpression phenomena of above-metioned three genes(MDR1、MRP、LRP)and their corresponding proteins(P-gp、MRP、LRP)was detected in the same lung cancer samples.There was close relationship between the expression of 3 genes and their corresponding proteins.The expression of MDR1 and its proten P-gp、 LRP mRNA in lung adenocarcinoma was higher than that in other pathological carcinomas(P0.05).The expression of MRPmRNA and its corresponding protein MRP was not related to pathological type、clinical stage and classification of histodifferentiation(P0.05).Conclusion:Multidrug resistance is more common in NSCLC.The genes of MDR1、MRP、 LRP mRNA and their corresponding proteins participated in the formation of multidrug resistance in lung cancer.Detection of multidrug resistance-related genes in lung cancer tissues may be useful to choice drugs.