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Content determination of the chlorogenic acid in Huangdanyinchen particles by HPLC

Lin Bi-ying

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Abstract

OBJECTIVE To establish the method of HPLC to determine the content of chlorogenic acid in Huangdanyinchen particles.METHODS HPLC was adopted by using Agilent TC-C18column(250mm×4.6mm,5μm).The mobile phase was acetonitrile-0.4% phosphonic acid(10∶90) with the detection wavelength 327nm,the sampling volume was 10μL,the flow rate was 1.0mL·min-1 and the temperature was 30℃.RESULTS There was a good linear relationship between the chlorogenic acid concentration and peak area within a range of 0.10μg~1.55μg(r=0.9997).The average recovery rate was 98.81%(RSD=0.45%,n=6).CONCLUSION The method is simple,accurate and can be used for the quality control of Huangdanyinchen particles.

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What this paper is about

OBJECTIVE To establish the method of HPLC to determine the content of chlorogenic acid in Huangdanyinchen particles.METHODS HPLC was adopted by using Agilent TC-C18column(250mm×4.6mm,5μm).The mobile phase was acetonitrile-0.4% phosphonic acid(10∶90) with the detection wavelength 327nm,the sampling volume was 10μL,the flow rate was 1.0mL·min-1 and the temperature was 30℃.RESULTS There was a good linear relationship between the chlorogenic acid concentration and peak area within a range of 0.10μg~1.55μg(r=0.9997).The average recovery rate was 98.81%(RSD=0.45%,n=6).CONCLUSION The method is simple,accurate and can be used for the quality control of Huangdanyinchen particles.

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Available abstract

OBJECTIVE To establish the method of HPLC to determine the content of chlorogenic acid in Huangdanyinchen particles.METHODS HPLC was adopted by using Agilent TC-C18column(250mm×4.6mm,5μm).The mobile phase was acetonitrile-0.4% phosphonic acid(10∶90) with the detection wavelength 327nm,the sampling volume was 10μL,the flow rate was 1.0mL·min-1 and the temperature was 30℃.RESULTS There was a good linear relationship between the chlorogenic acid concentration and peak area within a range of 0.10μg~1.55μg(r=0.9997).The average recovery rate was 98.81%(RSD=0.45%,n=6).CONCLUSION The method is simple,accurate and can be used for the quality control of Huangdanyinchen particles.

Key concepts: Chlorogenic acid, Chromatography, High-performance liquid chromatography, Chemistry, Linear relationship, Content determination, Linear range, Acetonitrile

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