Determination of chloramphenicol residue in chicken tissues by GC-μECD

A method of gas chromatography with microcell electron capture detector(GC-μECD) was developed to determine chloramphenicol residues in chicken muscle and liver tissues.The drug in tissues were extracted with ethyl acetate.The extract was defatted with hexane and purified with ethyl acetate by liquid-liquid distribution,(evaporated) to dryness with nitrogen gas.After derivatization by adding sylon BFT,tolune and water were added immediately to the sample.The organic layer was carefully removed.3μL processed liquid samples were determined by GC-μECD.Recoveries ranged from 82.9% to 94.3% with coefficients of variation from 4.5% to(14.5%).The limit of detection is 0.05 μg/kg in muscle,and 0.1 μg/kg in liver.