Determination of Chlorpromazine Residues in Chicken Muscle Tissues by High Performance Liquid Chromatography-tandem Mass Spectrometry

The study developed a qualitative and quantitative method of high performance liquid chromatography-tandemmass spectrometry for the determination of chlorpromazine residues in chicken muscle tissues.According to this method,thesamples were extracted with acidic acetonitrile and hexane,chromatographic separation was performed on C18 columnusing acetonitrile and 5 mmol/L ammonium acetate(containing 0.1% formic acid)in positive mode and acetonitrile andwater in negative mode,the samples were treated by electrospray ionization working with multi-reaction mode,quantifiedwith internal standard.The limit of detection of this method was 0.008μg/kg,limit of quantity was 0.025μg/kg,the recoveriesrate in chicken muscles were from 71.5% to 86.7% at 0.025 to 2.5μg/kg addition levels,intra-day and inter-day variablecoefficient was low than 7.6%.