Residues Detection of Dehydroacetic Acid in Chicken Tissues by High Performance Liquid Chromatography

A high performance liquid chromatography(HPLC)method was established to determine the residues of dehydroacetic acid(DHA)in chicken tissues.The tissue samples(muscle,liver and kidney)were extracted with acetonitrile,liquid-liquid extraction by hexane,purified by PAX solid phase extraction column.A C18 column with ultraviolet absorption detection at 290 nm,using methanol-water which includes 0.02% ammonium acetate(20/80,v/v)as eluent was used in HPLC.A linear calibration curve with coefficient relation(R2≥0.9996)in the range of 0.2 to 5.0 mg/L of DHA concentration was made.When DHA was added to tissues at dose of 0.5,1.0 and 5.0 mg/kg,the average recoveries in different tissues were ranged from 68.28% to 93.49%,with CV% ranged from 3.38% to 7.94% within-day and 4.31% to 7.80% between-day.The limit of detection and limit of quantitation of DHA were observed at 0.1 mg/kg and 0.2 mg/kg respectively.The established method was suitable for the determination of DHA residues in animal foods,be also useful to the investigation of DHA depletion,the maximum residue limit and its withdraw time.