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HPLC Simultaneous Determination of 7 Components in Shuanghuangqudu Tablets

Ziren Su

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Abstract

Objective:To establish a method for the determination of gallic acid,berberine hydrochloride,aloe emodin,rhein,emodin,chrysophanol and physcion in Shuanghuangqudu tablets.Method: The HPLC system consisted of a ZORBAX SB-Pheny Dimensions column(4.6 mm×15 cm,5 μm),methanol-0.1% phosphoric acid as mobile phase(using gradient) with flowing rate 1.0 mL·min-1 and detected at 310 nm.Result: The linear ranges of gallic acid,berberine hydrochloride,aloe emodin,rhein,emodin,chrysophanol and physcion were 0.12-2.4 μg(r=0.999 9),0.11-2.2 μg(r=0.999 9),0.015-0.3 μg(r=0.999 8),0.02-0.4 μg(r=0.999 8),0.008-0.16 μg(r=0.999 9),0.013-0.26 μg(r=0.999 8),0.006-0.12 μg(r=0.999 9),respectively,and the average recoveries(n=9) were 100.97%,100.20%,99.01%,99.76%,101.10%,101.97%,100.56%,respectively.Conclusion: The method is simple and repeatable,which can be applied to determine seven ingriedients at the same test condition in Shuanghuangqudu tablets.

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What this paper is about

Objective:To establish a method for the determination of gallic acid,berberine hydrochloride,aloe emodin,rhein,emodin,chrysophanol and physcion in Shuanghuangqudu tablets.Method: The HPLC system consisted of a ZORBAX SB-Pheny Dimensions column(4.6 mm×15 cm,5 μm),methanol-0.1% phosphoric acid as mobile phase(using gradient) with flowing rate 1.0 mL·min-1 and detected at 310 nm.Result: The linear ranges of gallic acid,berberine hydrochloride,aloe emodin,rhein,emodin,chrysophanol and physcion were 0.12-2.4 μg(r=0.999 9),0.11-2.2 μg(r=0.999 9),0.015-0.3 μg(r=0.999 8),0.02-0.4 μg(r=0.999 8),0.008-0.16 μg(r=0.999 9),0.013-0.26 μg(r=0.999 8),0.006-0.12 μg(r=0.999 9),respectively,and the average recoveries(n=9) were 100.97%,100.20%,99.01%,99.76%,101.10%,101.97%,100.56%,respectively.Conclusion: The method is simple and repeatable,which can be applied to determine seven ingriedients at the same test condition in Shuanghuangqudu tablets.

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Available abstract

Objective:To establish a method for the determination of gallic acid,berberine hydrochloride,aloe emodin,rhein,emodin,chrysophanol and physcion in Shuanghuangqudu tablets.Method: The HPLC system consisted of a ZORBAX SB-Pheny Dimensions column(4.6 mm×15 cm,5 μm),methanol-0.1% phosphoric acid as mobile phase(using gradient) with flowing rate 1.0 mL·min-1 and detected at 310 nm.Result: The linear ranges of gallic acid,berberine hydrochloride,aloe emodin,rhein,emodin,chrysophanol and physcion were 0.12-2.4 μg(r=0.999 9),0.11-2.2 μg(r=0.999 9),0.015-0.3 μg(r=0.999 8),0.02-0.4 μg(r=0.999 8),0.008-0.16 μg(r=0.999 9),0.013-0.26 μg(r=0.999 8),0.006-0.12 μg(r=0.999 9),respectively,and the average recoveries(n=9) were 100.97%,100.20%,99.01%,99.76%,101.10%,101.97%,100.56%,respectively.Conclusion: The method is simple and repeatable,which can be applied to determine seven ingriedients at the same test condition in Shuanghuangqudu tablets.

Key concepts: Emodin, Phosphoric acid, Chromatography, Chemistry, Gallic acid, High-performance liquid chromatography, Berberine hydrochloride, Methanol

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