Simultaneous determination of five anthraquinone constituents in Danning Tablets by HPLC

Objective:To simultaneously determine aloe-emodin,rhein,emodin,chrysophanol and physcion in Danning Tablets.Methods:HPLC was carried out on Shimadzu VP-ODS column(4.6 mm×250 mm,5.0μm)with acetonitrile-0.1%phosphoric acid as the mobile phase in gradient elution.The flow rate was 1.0 mL·min-1,the detection wavelength was 254 nm and the column temperature was 30℃.Results:Aloe-emodin,rhein,emodin,chrysophanol and physcion had good linear relationship in the range of 0.042～0.840μg(r= 0.9996),0.168～3.352μg(r=0.9998),0.084～1.680μg(r=0.9997),0.093～1.852μg(r=0.9999)and 0.174-3.488μg(r=0.9994).Their average recoveries were 99.8%(RSD=1.34%,n=5),99.8%(RSD=1.72%,n=5),100.0%(RSD=0.89%,n=5),99.5%(RSD=0.97%,n=5),and 100.2%(RSD=1.04%n=5),respectively.Conclusion:This method is rapid,simple and accurate,which can be suitable for the simultaneous determination of aloe-emodin,rhein,emodin,chrysophanol and physcion in Danning Tablets.