Qualitative and quantitive study of bax and bcl-2 protein expression in esophageal carcinona

Objective To explore the relationship between the abnormal Bax and Bcl 2 expression and carcinogenesis of esophageal carcinoma.Methods Bax and Bcl 2 protein expression were qualitatively and quantitatively detected using immunohistochemistry and flow cytomytry and celluar immunofluoresence technique from 70 cases of esophageal squamous cell carcinoma.Results The positive rate of Bax and Bcl 2 were 45.7% and 61.4% respectively by immunohistochemistry,Bax and Bcl 2 protein expression were well correlated with the pathological grading and lymph node metastasis(P0.05).The positive rate of Bax and Bcl 2 were 55.7% and 92.9% respectively by flow cytomytry and celluar immunofluoresence technique,Bax and Bcl 2 protein expression were not correlated with the pathological grading and staging and lymph node metastasis(P0.05).Conclusion Bax and Bcl 2 overexpression plays an important role in the development of esophageal carcinoma.