Determination of Matrine in Zhiyujing Mixtures by HPLC
Jin Tao
Abstract
Jin Tao
Abstract
Purpose To establish a HPLC methed for the determination of Matrine in Zhiyujing Mixtures.Methods The HPLC method was used at SunFire C18 column(4.6 mm×150 mm,3.5μm).The mobile phase consisted of methanol-water-triethylamine(48∶52∶0.05).The detective wavelength was 220 nm.Results The linearity of this method was good with the average recovery of 100.29%.Conclusion The method is simple and accurate.It can be used for the determination of the contents of Matrine in Zhiyujing Mixtures.
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Purpose To establish a HPLC methed for the determination of Matrine in Zhiyujing Mixtures.Methods The HPLC method was used at SunFire C18 column(4.6 mm×150 mm,3.5μm).The mobile phase consisted of methanol-water-triethylamine(48∶52∶0.05).The detective wavelength was 220 nm.Results The linearity of this method was good with the average recovery of 100.29%.Conclusion The method is simple and accurate.It can be used for the determination of the contents of Matrine in Zhiyujing Mixtures.
Key concepts: Matrine, Chromatography, Triethylamine, High-performance liquid chromatography, Chemistry, Linearity, Physics, Organic chemistry