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2001•Chinese Journal of Hospital PharmacyRequires access

Determination of matrine in Ganmeiling injection by HPLC

Yong Zhang

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Abstract

：OBJECTIVE To establish an HPLC method for determining matrine in Ganmeiling injection .METHODS The experimental conditions in the method were BDS-C18 column (5 μm, 250 mm×4 mm) and MeOH-water-triethylamine (50∶50∶0.2,adjusted to pH 9～10 with ammonia solution) as the mobile phase.The elute with a flow rate of 0.8 ml·min-1 was detected 220 nm. RESULTS The calibration curve was in good linearity over the range of 73～440 μg·ml -1(r=0.9993).The average recovery was 101.6%,RSD was 2.25 (n=3). CONCLUSIONS This HPLC method is simple,rapid,accurate,and is suitable for the quality contro l.

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What this paper is about

：OBJECTIVE To establish an HPLC method for determining matrine in Ganmeiling injection .METHODS The experimental conditions in the method were BDS-C18 column (5 μm, 250 mm×4 mm) and MeOH-water-triethylamine (50∶50∶0.2,adjusted to pH 9～10 with ammonia solution) as the mobile phase.The elute with a flow rate of 0.8 ml·min-1 was detected 220 nm. RESULTS The calibration curve was in good linearity over the range of 73～440 μg·ml -1(r=0.9993).The average recovery was 101.6%,RSD was 2.25 (n=3). CONCLUSIONS This HPLC method is simple,rapid,accurate,and is suitable for the quality contro l.

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Available abstract

：OBJECTIVE To establish an HPLC method for determining matrine in Ganmeiling injection .METHODS The experimental conditions in the method were BDS-C18 column (5 μm, 250 mm×4 mm) and MeOH-water-triethylamine (50∶50∶0.2,adjusted to pH 9～10 with ammonia solution) as the mobile phase.The elute with a flow rate of 0.8 ml·min-1 was detected 220 nm. RESULTS The calibration curve was in good linearity over the range of 73～440 μg·ml -1(r=0.9993).The average recovery was 101.6%,RSD was 2.25 (n=3). CONCLUSIONS This HPLC method is simple,rapid,accurate,and is suitable for the quality contro l.

Key concepts: Matrine, Chromatography, Calibration curve, Triethylamine, High-performance liquid chromatography, Chemistry, Detection limit, Organic chemistry

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