2014•Journal of Inner Mongolia Medical UniversityRequires access

EXTRACTION AND CONTENT DETERMINATION OF POLYSACCHARIDE IN MONGOLIAN MEDICINE

YU Shu-ya

Abstract

Objective: To explore the extraction and content determination of polysaccharide in the Mongolian medicine Terminalia chebula Retz.Methods: Phenol-sulfuric acid method and UV-PDA spectrophotometer were used to determine content of polysaccharide in Terminalia chebula Retz.With glucose as standard substance.Results: By the methodology study,polysaccharide had a linear relation within the scope of 3.212 to 19.27μg /mL,the regression equation was Y = 0.0432X + 0.0146(r= 0.9996,n = 6),the content was 15.56%,the average recovery was 100.4%,RSD = 1.60%.Conclusion: UV-Vis spectrophotometry is simple,rapid,sensitive,accurate and reliable,which can be used to determine polysaccharide in Mongolian medicine Terminalia chebula Retz.

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Key concepts: Terminalia chebula, Polysaccharide, Content determination, Extraction (chemistry), Chromatography, Chemistry, Traditional medicine, Sulfuric acid