2012•Zhongguo shiyan fangjixue zazhiRequires access

Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry

Liu Li-mei

Abstract

Objective:To develop a method for content determination of total polysaccharide in garlic polysaccharide.Method: Glucose was used as a reference substance,and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide.Result: The calibration curve of glucose showed good linearity in the range of 0.011 6-0.057 8 g·L-1(r=0.999 5).The average recovery rate was 101.4%,and RSD was 2.1%.Conclusion: The determination method is simple,sensitive and accurate,which can be used for evaluating the quality of total polysaccharide in the garlic polysaccharide.

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Key concepts: Polysaccharide, Colorimetry, Chemistry, Chromatography, Content determination, Recovery rate, Calibration curve, Reagent