2012•Journal of Inner Mongolia University for the NationalitiesRequires access

Determination on the Content of Polysaccharides in Mongolian Medicine Qingrebaweisan Crude Polysaccharide

Zhao Yu-ying

Abstract

Crude polysaccharide of the Mongolian medicine Qingrebaweisan was extracted by water extraction and alcohol precipitation method,and the content of polysaccharide was determined by the phenol-sulfuric acid method.The regression equation is A=23.1375C+0.0299,the related coefficient is 0.9992,and there were favorable linearity relationship between the absorption and content determined in the range of 20-100 μg/mL at the wavelength of 490 nm.The content of polysaccharides was about 56.86% with an average recovery of 100.09% and RSD of 0.96%.The phenol-sulfuric acid method can be used to determine the content of polysaccharide.

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Key concepts: Polysaccharide, Chromatography, Chemistry, Sulfuric acid, Content determination, Extraction (chemistry), Phenol, High-performance liquid chromatography