Expression and significance of cyclin D1,cyclin E and p27 in transitional cell carcinoma of the renal pelvis

Objective To investigate expression and clinical significance of cell cycle regulatory factor D1 ( cyclin D1),cell cycle regulatory factor E (cyclin E) and p27 in transitional cell carcinoma of the renal pelvis. Methods Expression levels of cyclin D1,cyclin E and p27 in surgical specimens from 63 patients were examined by immunohistochemistry. Pathological grading showed a G1 tumor in 12 cases,G2 in 40 and G3 in 11; TNM staging showed a Ta tumor in 4 cases,T1 in 9,T2 in 24,T3 in 17 and T4 in 9. Eleven specimens obtained from normal renal pelvises served as the controls. Results Cyclin D1 was weakly expressed and cyclin E was not expressed while p27 was strongly positive in normal pelvic tissue. In transitional cell carcinoma,expressions of cyclin D1 and cyclin E were significantly increased (P 0. 05),while expression of p27 was significantly decreased (P 0. 05). With the increasing of grades and stages in transitional cell carcinoma of renal pelvis,expression rates of cyclin D1 and cyclin E increased while expression rate of p27 decreased (P 0. 05),and p27 expression was negatively related with cyclin D1 and cyclin E expression respectively(P 0. 05). Conclusion Cyclin D1 and cyclin E over-expressions and p27 deactivation are found in transitional cell carcinoma of the renal pelvis. Combined detections of cyclin D1,cyclin E and p27 may help reveal the malignancy of transitional cell carcinoma and predict the prognosis.