2004•China Surfactant Detergent & CosmeticsRequires access

Determination of amentoflavone by high performance liquid chromatography

LI Wu-zhou, Jim Jian Wang

Abstract

A method for determination of amentoflavone in extract of Selaginella tamariscina by high performance liquid chromatography was set up.Chromatographic analysis was carried out on a Kromasil C_(18) columm with methanol/0.1 % H_3PO_4 aqueous solution as mobile phase,and the flow rate was 0.80 mL/min.The detection wavelength was 337 nm.The linear relationship of calibration curve of amentoflavone is good in the range of 41.2 mg/L～206 mg/L,and the regression equation was Y=547.028X+15.489 5(r=0.999 9).The average recovery of amentoflavone was 99.42 %(n=3) and the RSD was 0.79 %.

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Key concepts: Amentoflavone, Chromatography, Calibration curve, Chemistry, High-performance liquid chromatography, Analytical Chemistry (journal), Detection limit